The development of the hotel cost £25m and was designed to reflect Southampton’s marine culture as well as offer a gateway to Solent.
The hotel features a rooftop destination bar with views of the marina and a restaurant on the ground floor, which serves local and seasonal seafood.
It also has a cinema which seats 21 people and an outdoor terrace with fire pits and a wood fired oven. The Southampton Harbour Hotel has an event space on the first floor with a capacity for 200 guests.
The hotel will also feature a further six apartments.
Mike Warren, managing director of Harbour Hotels, said: “We are thrilled to unveil our spectacular new Southampton Harbour Hotel & Spa development at Ocean Village, introducing five-star luxury to the marina.
“Attracting tourism and providing a number of fantastic dining experiences, an indulgent spa and Southampton’s first destination rooftop bar for locals to enjoy, we are very excited to officially open our doors.”