Detectives investigating believe that both victims, who are in their late 30s/early 40s and from the London area, were known to each other.
They have confirmed that they are not looking for anyone else in connection with the deaths.
According to police, the two bodies were discovered at the Doubletree by Hilton Harrogate Majestic Hotel and Spa on Springfield Avenue at 10.20pm on Tuesday 4 May 2021.
Wayne Fox, detective superintendent of North Yorkshire Police’s Major Investigation Team said: “We were called at around 10.20pm on Tuesday 4 May by hotel staff at the Majestic Hotel to a report that the bodies of a man and a woman had been located in a hotel room.
“On arrival, a man and a woman, both in their late 30s/early 40s from the London area, were pronounced dead at the scene by colleagues from the ambulance service. Their families have been informed and are being supported by specially-trained officers.
He added: “A detailed investigation is underway looking into the circumstances of both deaths. We can confirm that we are not looking for anyone else in connection with the deaths and would like to reassure the local community that we believe that this is an isolated incident.”